Considerations To Know About mif
Considerations To Know About mif
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The mitochondrial membrane possible was evaluated utilizing the fluorescent probe TMRM dye (Invitrogen) as per the manufacturer’s Guidelines.
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The Glide module in Schrödinger was utilized for molecular docking calculations [28]. The tautomers and protonation states of the ligands ended up produced at pH =7.four by LigPrep in Schrödinger after which you can docked towards the binding web page of MIF by Glide in excess precision (XP) scoring method. The bounding box was centered about the cocrystallized ligand.
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